PMC

Set-up for different fields of view. (a) Desired field of view of 3072 pixel diameter. (b) Wide-field scanning protocol for covering the desired field of view of panel (a) with merged projections from one central and two half ring scans (r ₁ and r ₂). (c) Desired field of view of 5120 pixel diameter. (d) Wide-field scanning protocol for covering the desired field of view of panel (c) with merged projections from one central and four half ring scans (r ₁–r ₄). (e) Desired field of view of 7168 pixel diameter. (f) Wide-field scanning protocol for covering the desired field of view of panel (e) with merged projections from one central and six half ring scans (r ₁–r ₆).

(a) Input image of matrix size 1024 × 1024 displayed at 50% while the extracted foreground image (643 × 887) displayed at 100%. (b) The extracted useful field of view (579 × 799) and extracted central field of view images (435 × 599) from the input image (1024 × 1024) as shown in Figure 3a

A–C: Field of view immediately adjacent to the denuded region of the RPE double-labeled with anti-GFP and RPE-65. The denuded region is just superior to and left of the area shown. A: GFP⁺ RPE cells are evident throughout this field of view. B: All cells in this field of view are RPE-65⁺, confirming that GFP⁺ cells are RPE cells. C: Co-localization of GFP and RPE-65 show that the two markers co-localize to the same population. D–F: Field of view at the edge of the eyecup showing far fewer GFP⁺ RPE cells double-labeled with anti-GFP and RPE-65. RPE morphology of host-derived RPE cells away from the wound shows a much smaller nuclear size compared to GFP⁺ HSC-derived RPE cells. D: Very few GFP⁺ RPE cells are evident throughout this field of view. E: All cells in this field of view are RPE-65⁺, confirming that GFP⁺ cells are RPE cells. F: Co-localization of GFP and RPE-65 shows that the two markers co-localize to the same population. G–I: Field of view adjacent to the denuded region of the RPE double-labeled with anti-GFP and cytokeratin 8/18. The denuded region is just superior to and left of the area shown. Cytokeratin bundles outline the periphery of cuboidal RPE cells. The nuclei and larger melanin granules impart a finely vacuolated appearance to the less intensely immunopositive central cytoplasm. G: GFP⁺ RPE cells are evident throughout this field of view with a small number of GFP⁻ cells evident in the lower left area of the field of view. H: Co-localization of GFP and cytokeratin 8/18 show that the two markers co-localize to the same population.

(a) Originally measured uncorrected sinogram, (b) small FOV based sinogram, (c,e) sinograms with the previous LMAR and NMAR methods, and (d,f) sinograms with the proposed LMAR and NMAR methods. The red dotted lines in the sinogram images represent the central profiles at the 189th view, and the gray shading in the central profiles represent the metal trace regions. The blue lines in the plots represent the sinogram values at the 189th view, and the red lines represent the reference sinogram values at the 189th view.